|
KMID : 0620920070390020139
|
|
Experimental & Molecular Medicine
2007 Volume.39 No. 2 p.139 ~ p.148
|
|
|
Curcumin reduces cold storage-induced damage in human cardiac myoblasts
|
|
Abuarqoub Hadil
Green Colin J.
Foresti Roberta
Motterlini Roberto
|
|
|
Abstract
|
|
|
|
Curcumin is a polyphenolic compound possessing interesting anti-inflammatory and antioxidant properties and has the ability to induce the defensive protein heme oxygenase-1 (HO-1). The objective of this study was to investigate whether curcumin protects against cold storage-mediated damage of human adult atrial myoblast cells (Girardi cells) and to assess the potential involvement of HO-1 in this process. Girardi cells were exposed to either normothermic or hypothermic conditions in Celsior preservation solution in the presence or absence of curcumin. HO-1 protein expression and heme oxygenase activity as well as cellular damage were assessed after cold storage or cold storage followed by re-warming. In additional experiments, an inhibitor of heme oxygenase activity (tin protoporphyrin IX, 10 mM) or siRNA for HO-1 were used to investigate the participation of HO-1 as a mediator of curcumin- induced effects. Treatment with curcumin produced a marked induction of cardiac HO-1 in normothermic condition but cells were less responsive to the polyphenolic compound at low temperature. Cold storage-induced damage was markedly reduced in the presence of curcumin and HO-1 contributed to some extent to this effect. Thus, curcumin added to Celsior preservation solution effectively prevents the damage caused by cold- storage; this effect involves the protective enzyme HO-1 but also other not yet identified mechanisms.
|
|
|
KEYWORD
|
|
|
cryopreservation curcumin, cytoprotection, heme oxygenase-1, organ preservation, organ transplantation, oxidative stress
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|